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Antikörper

CLOCK Rabbit polyclonal Antibody

Produktgrößen
100μl
£370,00
R1511-2-100UL
Über dieses Produkt
SKU:
R1511-2
Anwendung:
Western Blot, IHC-Paraffin, Flow Cytometry
Buffer:
1*PBS (pH7.4); 0.2% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
Klonalität:
Polyclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within mouse CLOCK 829-855/855.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 95 kDa
Physischer Zustand:
Liquid
Reinheit:
Immunogen affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Polyclonal Antibody
Western blot analysis of CLOCK on different lysates with Rabbit anti-CLOCK antibody (R1511-2) at 1/1;000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: MCF7 cell lysate (10 µg/Lane) Lane 3: HEK-293 cell lysate (10 µg/Lane) Lane 4: NIH/3T3 cell lysate (10 µg/Lane) Lane 5: PC-12 cell lysate (10 µg/Lane) Lane 6: Human brain tissue lysate (20 µg/Lane) Lane 7: Mouse brain tissue lysate (20 µg/Lane) Lane 8: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 95 kDa Observed band size: 95 kDa Exposure time: 13 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1511-2) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunocytochemical staining of PC-3M cells using anti-CLOCK rabbit polyclonal antibody.
Immunocytochemical staining of SW480 cells using anti-CLOCK rabbit polyclonal antibody.
Immunocytochemical staining of Hela cells using anti-CLOCK rabbit polyclonal antibody.