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Antikörper

Alpha-2-macroglobulin Mouse monoclonal Antibody

Produktgrößen
100μl
£400,00
M1510-22-100UL
Über dieses Produkt
SKU:
M1510-22
Anwendung:
Western Blot, IHC-Paraffin, ELISA
Buffer:
1*PBS (pH7.4); 0.2% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
A5-A9
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within human Alpha-2-macroglobulin aa 950-1200.
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 163 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein G affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Alpha-2-macroglobulin on LOVO cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1510-22; 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Alpha-2-macroglobulin on LO2 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1510-22; 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5;000 dilution was used for 1 hour at room temperature.
ICC staining of Alpha-2-macroglobulin in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1510-22; 1/50) for 1 hour at room temperature; washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1;000 dilution. The nuclear counter stain is DAPI (blue).
ICC staining of Alpha-2-macroglobulin in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1510-22; 1/100) for 1 hour at room temperature; washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1;000 dilution. The nuclear counter stain is DAPI (blue).