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Antikörper

CHD1 Mouse monoclonal Antibody

Produktgrößen
100μl
£400,00
M1211-5-100UL
Über dieses Produkt
SKU:
M1211-5
Anwendung:
Western Blot, IHC-Paraffin
Buffer:
1*PBS (pH7.4); 0.2% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
2-2
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within Human CHD1 aa 1;479-1;710 / 1;710.
Isotyp:
IgG1
Molekulargewicht:
Predicted band size: 197 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of CHD1 on different lysates with Mouse anti-CHD1 antibody (M1211-5) at 1/5;000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MOLT-4 cell lysate Lane 4: COS-1 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 197 kDa Observed band size: 260 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-5) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of CHD1 on different lysates with Mouse anti-CHD1 antibody (M1211-5) at 1/1;000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-CHD1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 197 kDa Observed band size: 260 kDa Exposure time: 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1211-5) at 1/1;000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis on recombinant protein using anti-CHD1 Mouse mAb (Cat. # M1211-5).
Immunohistochemical analysis of paraffin- embedded mouse testis tissue using anti-CHD1 Mouse mAb (Cat. # M1211-5).