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Antikörper

GRAMD1A Mouse monoclonal Antibody

Produktgrößen
100μl
£400,00
M1010-3-100UL
Über dieses Produkt
SKU:
M1010-3
Anwendung:
Western Blot, IHC-Paraffin
Buffer:
1*PBS (pH7.4); 0.2% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
1-E4
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Recombinant protein within Human GRAMD1A aa 1-724 / 724.
Isotyp:
IgM
Molekulargewicht:
Predicted band size: 81 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of GRAMD1A on NIH/3T3 (1) and SHG-44 (2) cells lysates using anti-GRAMD1A antibody at 1/1;000 dilution.
Immunocytochemistry analysis of A549 cells labeling GRAMD1A with Mouse anti-GRAMD1A antibody (M1010-3) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes; and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-GRAMD1A antibody (M1010-3) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488; HA1125) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-GRAMD1A antibody (M1010-3) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (M1010-3) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Mouse anti-GRAMD1A antibody (M1010-3) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (M1010-3) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.