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Antikörper

Synaptophysin Mouse monoclonal Antibody

Produktgrößen
100μl
£400,00
M0407-30-100UL
Über dieses Produkt
SKU:
M0407-30
Anwendung:
Western Blot, IHC-Paraffin, Flow Cytometry
Buffer:
1*PBS (pH7.4); 0.2% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
A1-7
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Mouse
Immunogen:
Synthetic peptide (KLH-coupled) within human Synaptophysin 220-280 aa.
Isotyp:
IgG1
Molekulargewicht:
34kDa
Physischer Zustand:
Liquid
Reinheit:
Immunogen affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Synaptophysin on mouse brain tissue lysate using anti-Synaptophysin antibody at 1/2;000 dilution.
ICC staining Synaptophysin (red) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde; permeabilised with 0.25% Triton X100/PBS.
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-Synaptophysin antibody (M0407-30) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (M0407-30) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded medullary thyroid carcinoma tissue with Mouse anti-Synaptophysin antibody (M0407-30) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (M0407-30) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.