Western blot analysis of MMP-1 on different lysates with Rabbit anti-MMP-1 antibody (ER31211) at 1/1;000 dilution. Lane 1: SK-Br-3 cell lysate (15 µg/Lane) Lane 2: MCF7 cell lysate (15 µg/Lane) Lane 3: Raji cell lysate (15 µg/Lane) Lane 4: A431 cell lysate (15 µg/Lane) Lane 5: HeLa cell lysate (15 µg/Lane) Lane 6: HUVEC cell lysate (15 µg/Lane) Lane 7: A549 cell lysate lysate (30 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER31211) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100;000 dilution was used for 1 hour at room temperature.
Western blot analysis of MMP-9 on different lysates with Rabbit anti-MMP-9 antibody (ET1704-69) at 1/5;000 dilution. Lane 1: THP-1 whole cell lysate (20 µg/Lane) Lane 2: THP-1 treated with 200nM TPA for 10 minutes whole cell lysate (20 µg/Lane) Lane 3: Rat lung tissue lysate (20 µg/Lane) Lane 4: Rat spleen tissue lysate (20 µg/Lane) Predicted band size: 78 kDa Observed band size: 92 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1704-69) at 1/5;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100;000 dilution was used for 1 hour at room temperature.
Western blot analysis of MMP-13 on different lysates with Rabbit anti-MMP-13 antibody (ET1702-14) at 1/2;000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: Jurkat cell lysate (15 µg/Lane) Lane 4: MDA-MB-231 cell lysate (15 µg/Lane) Lane 5: SW480 cell lysate (15 µg/Lane) Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-14) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Fibronectin on different lysates with Rabbit anti-Fibronectin antibody (ET1702-25) at 1/2;000 dilution and competitor's antibody at 1/2;000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: Caco-2 cell lysate (20 µg/Lane) Lane 3: SK-MEL-28 cell lysate (20 µg/Lane) Lane 4: Human kidney tissue lysate (20 µg/Lane) Predicted band size: 272 kDa Observed band size: 272 kDa Exposure time: 3 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-25) at 1/2;000 dilution and competitor's antibody at 1/2;000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
