All lanes: Western blot analysis of Integrin alpha V with anti-Integrin alpha V antibody (ET1610-15) at 1/500 dilution. Lane 1: Wild-type A549 whole cell lysate. Lane 2/3: Integrin alpha V knockdown A549 whole cell lysate. ET1610-15 was shown to specifically react with Integrin alpha V in Wild-type A549 cells. Weakened bands were observed when Integrin alpha V knockdown samples were tested. Wild-type and Integrin alpha V knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Integrin alpha V antibody (ET1610-15; 1/500) and Anti-HSP90 antibody (ET1605-56; 1/10;000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:300;000 dilution was used for 1 hour at room temperature.
