Western blot analysis of ATG5 on different lysates with Rabbit anti-ATG5 antibody (ET1611-38) at 1/5;000 dilution and competitor's antibody at 1/1;000 dilution. Lane 1: NIH/3T3 cell lysate (15 µg/Lane) Lane 2: C2C12 cell lysate (15 µg/Lane) Lane 3: Neuro-2a cell lysate (15 µg/Lane) Lane 4: PC-12 cell lysate (15 µg/Lane) Lane 5: Mouse brain tissue lysate (15 µg/Lane) Lane 6: Rat brain tissue lysate (15 µg/Lane) Predicted band size: 32 kDa Observed band size: 55 kDa Exposure time: 35 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-38) at 1/5;000 dilution and competitor's antibody at 1/1;000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATG12 on different lysates with Rabbit anti-ATG12 antibody (HA721504) at 1/1;000 dilution. Lane 1: HeLa cell lysate; 20 µg/Lane Lane 2: HCT 116 cell lysate; 20 µg/Lane Lane 3: HEK-293 cell lysate; 20 µg/Lane Lane 4: SH-SY5Y cell lysate; 20 µg/Lane Predicted band size: 15 kDa Observed band size: 55/20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721504) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Atg16L1 on different lysates with Rabbit anti-Atg16L1 antibody (ET7106-65) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 68 kDa Observed band size: 68/66 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-65) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
