Western blot analysis of ALIX on different lysates with Rabbit anti-ALIX antibody (ET1705-74) at 1/2;000 dilution. Lane 1: HEK-293 cell lysate Lane 2: K-562 cell lysate Lane 3: Jurkat cell lysate Lane 4: PC-3M cell lysate Lane 5: MCF7 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 96 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-74) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (ET1601-9) at 1/1;000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: HCT 116 cell lysate (12 µg/Lane) Lane 5: HepG2 cell lysate (20 µg/Lane) Lane 6: Jurkat cell lysate (20 µg/Lane) Lane 7: Raji cell lysate (20 µg/Lane) Lane 8: Rat lung tissue lysate (20 µg/Lane) Predicted band size: 25 kDa Observed band size: 23 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-9) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Hsp70 on different lysates with Rabbit anti-Hsp70 antibody (ET1601-11) at 1/2;000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: MCF7 cell lysate Lane 4: HCT 116 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: Lane 1-4: 43 seconds; Lane 5-7: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-11) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50;000 dilution was used for 1 hour at room temperature.
