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Antikörper

Acid sphingomyelinase Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£425,00
HA751572-100UL
Über dieses Produkt
SKU:
HA751572
Anwendung:
Western Blot, IHC-Paraffin, Immunoprecipitation
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH15-61
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human Acid sphingomyelinase aa 47-631.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 70 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Acid sphingomyelinase on different lysates with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/5;000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300n/mL BFA for 3 hours cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: HepG2 treated with 10ng/mL BFA for 1.5 hours cell lysate (20 µg/Lane) Lane 5: MOLT-4 cell lysate (low expression) (20 µg/Lane) Lane 6: COS-1 cell lysate (20 µg/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751572) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Acid sphingomyelinase on different lysates with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/5;000 dilution. Lane 1: PC-12 cell lysate (40 µg/Lane) Lane 2: PC-12 starved for 16 hours then treated with 200nM TPA for 4 hours cell lysate (40 µg/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 18 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751572) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Acid sphingomyelinase on RAW264.7 cell lysates with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/2;000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751572) at 1/2;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Acid sphingomyelinase antibody (HA751572) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA751572) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.