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Antikörper

ALP Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA751517-100UL
Über dieses Produkt
SKU:
HA751517
Anwendung:
Western Blot, Immunoprecipitation
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH14-35
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human ALP aa 1-364.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 39 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of ALP on different lysates with Rabbit anti-ALP antibody (HA751517) at 1/5;000 dilution. Lane 1: Saos-2 cell lysate Lane 2: RD cell lysate Lane 3: U-2 OS cell lysate Lane 4: 293T cell lysate Lane 5: C2C12 cell lysate Lane 6: L6 cell lysate Lane 7: Mouse heart tissue lysate Lane 8: Mouse kidney tissue lysate (negative) Lane 9: Rat heart tissue lysate Lane 10: Rat kidney tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 39 kDa Observed band size: 35 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751517) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
ALP was immunoprecipitated from 0.2 mg Saos-2 cell lysate with HA751517 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751517 at 1/5;000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5;000 dilution was used for 1 hour at room temperature. Lane 1: Saos-2 cell lysate (input) Lane 2: HA751517 IP in Saos-2 cell lysate Lane 3: Rabbit IgG instead of HA751517 in Saos-2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 46 seconds; ECL: K1801