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Antikörper

BATF3 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA751471-100UL
Über dieses Produkt
SKU:
HA751471
Anwendung:
Western Blot, Immunoprecipitation, ChIP
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH13-52
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human BATF3 aa 1-127.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 14 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of BATF3 on different lysates with Rabbit anti-BATF3 antibody (HA751471) at 1/5;000 dilution. Lane 1: U-937 cell lysate Lane 2: Ramos cell lysate (negative) Lane 3: HDLM2 cell lysate Lane 4: HepG2 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 14 kDa Observed band size: 18 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751471) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
BATF3 was immunoprecipitated from 0.2 mg U-937 cell lysate with HA751471 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751471 at 1/5;000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5;000 dilution was used for 1 hour at room temperature. Lane 1: U-937 cell lysate (input) Lane 2: HA751471 IP in U-937 cell lysate Lane 3: Rabbit IgG instead of HA751471 in U-937 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 3 minutes; ECL: K1801
Chromatin immunoprecipitations were performed with cross-linked chromatin from U-937 cells with BATF3 (HA751471) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin; which is equivalent to one.