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Antikörper

ROR alpha / RORA Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA751390-100UL
Über dieses Produkt
SKU:
HA751390
Anwendung:
IHC-Paraffin, Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH11-00
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within mouse RORA aa aa 101-523.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 59 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-ROR alpha / RORA antibody (HA751390) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA751390) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue (negative) with Rabbit anti-ROR alpha / RORA antibody (HA751390) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA751390) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of ROR alpha / RORA on different lysates with Rabbit anti-ROR alpha / RORA antibody (HA751390) at 1/2;000 dilution. Lane 1: Mouse brain tissue lysate (40 µg/Lane) Lane 2: Mouse cerebellum tissue lysate (40 µg/Lane) Lane 3: Mouse spleen tissue lysate (negative) (40 µg/Lane) Lane 4: Rat cerebellum tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 59 kDa Observed band size: 56/59 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751390) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.