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Antikörper

Arc / Arg3.1 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA751369-100UL
Über dieses Produkt
SKU:
HA751369
Anwendung:
Western Blot, IHC-Paraffin, IHC-Frozen
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH10-67
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 45 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Application: IHC-Fr Species: Mouse Site: Hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Arc / Arg3.1 antibody (HA751369) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA751369) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Arc / Arg3.1 antibody (HA751369) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA751369) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Arc / Arg3.1 on different lysates with Rabbit anti-Arc / Arg3.1 antibody (HA751369) at 1/2;000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse kidney tissue lysate (low expression) Lane 3: Rat brain tissue lysate Lane 4: Rat kidney tissue lysate (low expression) Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 50 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751369) at 1/2;000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.