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Antikörper

Aquaporin 2 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA751273-100UL
Über dieses Produkt
SKU:
HA751273
Anwendung:
IHC-Paraffin, IHC-Frozen
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH09-19
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human Aquaporin 2 aa 215-263/271
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 29 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Immunofluorescence analysis of frozen mouse kidney tissue with Rabbit anti-Aquaporin 2 antibody (HA751273) at 1/5;000 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature; washed with PBS; and then probed with the primary antibody (HA751273; green) at 1/5;000 dilution overnight at 4 ℃; washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. Nuclei were counterstained with DAPI (blue).
Immunofluorescence analysis of frozen rat kidney tissue with Rabbit anti-Aquaporin 2 antibody (HA751273) at 1/800 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature; washed with PBS; and then probed with the primary antibody (HA751273; green) at 1/800 dilution overnight at 4 ℃; washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. Nuclei were counterstained with DAPI (blue).
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Aquaporin 2 antibody (HA751273) at 1/100;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA751273) at 1/100;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-Aquaporin 2 antibody (HA751273) at 1/100;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA751273) at 1/100;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.