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Antikörper

ASPA Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA751204-100UL
Über dieses Produkt
SKU:
HA751204
Anwendung:
Western Blot, IHC-Paraffin, IHC-Frozen
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH08-04
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human ASPA aa 1-313.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 36 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS; pH 7.4) for 5 minutes at room temperature.
Western blot analysis of ASPA on different lysates with Rabbit anti-ASPA antibody (HA751204) at 1/5;000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse kidney tissue lyaste Lane 4: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 36 kDa Observed band size: 35 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751204) at 1/5;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of ASPA on different lysates with Rabbit anti-ASPA antibody (HA751204) at 1/5;000 dilution. Lane 1: 293T transfected with empty control cell lysate Lane 2: 293T transfected with human ASPA cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751204) at 1/5;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.