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Antikörper

CXCL1 / GRO alpha Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA751174-100UL
Über dieses Produkt
SKU:
HA751174
Anwendung:
Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH07-53
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human CXCL1 aa 1-107.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 11 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of CXCL1 / GRO alpha on different lysates with Rabbit anti-CXCL1 / GRO alpha antibody (HA751174) at 1/2;000 dilution. Lane 1: HUVEC cell lysate Lane 2: HUVEC treated with 0.5μg/mL LPS for 24 hours cell lysate; then add 300ng/mL BFA for 20 hours cell lysate Lane 3: EA.hy926 cell lysate Lane 4: EA.hy926 treated with 0.5μg/mL LPS for 4 hours cell lysate; then add 300ng/mL BFA for 20 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 11 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722841) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.