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Antikörper

Phospho-STAT6 (Y641) Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA751125-100UL
Über dieses Produkt
SKU:
HA751125
Anwendung:
Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH06-98
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Tyr641 of human p53 aa 611-660..
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 94 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA751125) at 1/1;000 dilution. Lane 1: Daudi cell lysate Lane 2: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate Lane 3: Daudi treated with 100ng/mL hIL-4 for 15 minutes cell lysate; then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 94 kDa Observed band size: 100 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751125) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-STAT6 (Y641) on different lysates with Rabbit anti-Phospho-STAT6 (Y641) antibody (HA751125) at 1/2;000 dilution. Lane 1: J774A.1 cell lysate Lane 2: J774A.1 treated with 10ng/mL mIL-4 for 2 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 94 kDa Observed band size: 100 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751125) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.