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Antikörper

Phospho-p38 alpha (T180 + Y182) Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA751074-100UL
Über dieses Produkt
SKU:
HA751074
Anwendung:
Western Blot, Flow Cytometry
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH06-35
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 alpha / MAPK14.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 41 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-p38 alpha (T180 + Y182) on different lysates with Rabbit anti-Phospho-p38 alpha (T180 + Y182) antibody (HA751074) at 1/2;000 dilution and p38 alpha antibody (ET1702-65) at 1/1;000 dilution. Lane 1: Jurkat cell lysate (20 µg/Lane) Lane 2: Jurkat treated with UV for 1 hour cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 treated with 25μg/mL Anisomycin for 30 minutes cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: C6 treated with 25μg/mL Anisomycin for 30 minutes cell lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751074) at 1/2;000 dilution and p38 alpha antibody (ET1702-65) at 1/1;000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-p38 alpha (T180 + Y182) on different lysates with Rabbit anti-Phospho-p38 alpha (T180 + Y182) antibody (HA751074) at 1/1;000 dilution. Lane 1: PC-12 cell lysate (20 µg/Lane) Lane 2: PC-12 treated with 25μg/mL Anisomycin for 30 minutes cell lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 3 minute 30 secons; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751074) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Jurkat cells treated with UV for 1 hour labeling Phospho-p38 alpha (T180 + Y182) with Rabbit anti-Phospho-p38 alpha (T180 + Y182) antibody (HA751074) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-p38 alpha (T180 + Y182) antibody (HA751074) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.
Immunocytochemistry analysis of NIH/3T3 cells treated with 25μg/mL Anisomycin for 30 minutes labeling Phospho-p38 alpha (T180 + Y182) with Rabbit anti-Phospho-p38 alpha (T180 + Y182) antibody (HA751074) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-p38 alpha (T180 + Y182) antibody (HA751074) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.