Skip to content

Warenkorb

Der Warenkorb ist leer.

Gesamt (exkl. MwSt.) £0,00
Warenkorb überprüfen & bezahlen
Antikörper

Calsequestrin 2 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750857-100UL
Über dieses Produkt
SKU:
HA750857
Anwendung:
Western Blot, IHC-Paraffin
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH03-16
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human Calsequestrin 2 aa 1-399 / 399.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 46 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Calsequestrin 2 on different lysates with Rabbit anti-Calsequestrin 2 antibody (HA750857) at 1/10;000 dilution and competitor's antibody at 1/5;000 dilution. Lane 1: Mouse skeletal muscle tissue lysate (15 µg/Lane) Lane 2: Mouse heart tissue lysate (15 µg/Lane) Lane 3: Mouse brain tissue lysate (negative) (15 µg/Lane) Lane 4: Rat skeletal muscle tissue lysate (15 µg/Lane) Lane 5: Rat heart tissue lysate (15 µg/Lane) Lane 6: Rat brain tissue lysate (negative) (15 µg/Lane) Predicted band size: 46 kDa Observed band size: 55 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750857) at 1/10;000 dilution and competitor's antibody at 1/5;000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunofluorescence analysis of paraffin-embedded human skeletal muscle tissue labeling Calsequestrin 2 with Rabbit anti-Calsequestrin 2 antibody (HA750857) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature; washed with PBS; and then probed with the primary antibody (HA750857; green) at 1/500 dilution overnight at 4 ℃; washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. Nuclei were counterstained with DAPI (blue).
Immunofluorescence analysis of paraffin-embedded mouse skeletal muscle tissue labeling Calsequestrin 2 with Rabbit anti-Calsequestrin 2 antibody (HA750857) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature; washed with PBS; and then probed with the primary antibody (HA750857; green) at 1/500 dilution overnight at 4 ℃; washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. Nuclei were counterstained with DAPI (blue).
Immunofluorescence analysis of paraffin-embedded rat skeletal muscle tissue labeling Calsequestrin 2 with Rabbit anti-Calsequestrin 2 antibody (HA750857) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature; washed with PBS; and then probed with the primary antibody (HA750857; green) at 1/500 dilution overnight at 4 ℃; washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. Nuclei were counterstained with DAPI (blue).