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Antikörper

Axin1 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750855-100UL
Über dieses Produkt
SKU:
HA750855
Anwendung:
Western Blot, IHC-Paraffin
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH03-00
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human Axin1 aa 1-400 / 862.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 95.6 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Axin1 on different lysates with Rabbit anti-Axin1 antibody (HA750855) at 1/2;000 dilution. Lane 1: SW480 cell lysate (10 µg/Lane) Lane 2: HeLa cell lysate (10 µg/Lane) Lane 3: 293T cell lysate (10 µg/Lane) Lane 4: K-562 cell lysate (10 µg/Lane) Lane 5: Huh7 cell lysate (10 µg/Lane) Predicted band size: 96 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750855) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Axin1 on different lysates with Rabbit anti-Axin1 antibody (HA750855) at 1/2;000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Axin1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 96 kDa Observed band size: 110 kDa Exposure time: 140 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750855) at 1/2;000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-Axin1 antibody (HA750855) at 1/2;000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA750855) at 1/2;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.