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Antikörper

ZIP Kinase Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750788-100UL
Über dieses Produkt
SKU:
HA750788
Anwendung:
Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH02-18
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Human ZIP Kinase aa 250-454 (O43293).
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 53 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of ZIP Kinase on different lysates with Rabbit anti-ZIP Kinase antibody (HA750788) at 1/1;000 dilution. Lane 1: A431 cell lysate Lane 2: A549 cell lysate Lane 3: 293T cell lysate Lane 4: SiHa cell lysate Lane 5: HepG2 cell lysate Lane 6: Hela cell lysate Lane 7: Jurkat cell lysate Lane 8: Caco-2 cell lysate Lane 9: K-562 cell lysate Lane 10: MCF7 cell lysate Lane 11: U-2 OS cell lysate Lane 12: Human kidney tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 53 kDa Observed band size: 50 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750788) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of ZIP Kinase on different lysates with Rabbit anti-ZIP Kinase antibody (HA750788) at 1/2;000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si ZIP Kinase cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750788) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.