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Antikörper

Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750717-100UL
Über dieses Produkt
SKU:
HA750717
Anwendung:
Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH01-39
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within human PIK3R1 aa 451-500 / 724.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 84 kDa/55 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) on different lysates with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody (HA750717) at 1/1;000 dilution. Lane 1: NIH/3T3 whole cell lysate Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 84 kDa Observed band size: 55/85 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750717) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) on different lysates with Rabbit anti-Phospho-PI3K p85 (Y467) + PI3K p55 (Y199) antibody (HA750717) at 1/1;000 dilution. Lane 1: 293T overexpress Src whole cell lysate Lane 2: 293T overexpress Src treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate Lane 3: 293T overexpress Src treated with λpp for 1 hour whole cell lysate Lane 4: 293T overexpress Src treated with 1mM sodium orthovanadate for 30 minutes; then treated with λpp for 1 hour whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 55 kDa Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750717) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100;000 dilution was used for 1 hour at room temperature.