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Antikörper

NDP52 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750623-100UL
Über dieses Produkt
SKU:
HA750623
Anwendung:
Western Blot, Flow Cytometry
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH02-33
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human NDP52 aa 1-150 / 446 (Q13137).
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 52 kDa
Physischer Zustand:
Liquid
Reinheit:
Immunogen affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of NDP52 on different lysates with Rabbit anti-NDP52 antibody (HA750623) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: Raji cell lysate Lane 4: THP-1 cell lysate Lane 5: Huh7 cell lysate Lane 6: HepG2 cell lysate Lane 7: Saos-2 cell lysate Lane 8: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750623) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of HeLa cells labeling NDP52. Cells were fixed and permeabilized. Then stained with the primary antibody (HA750623; 1/1;000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour; the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1;000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).