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Antikörper

Phospho-ATR (T1989) Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750524-100UL
Über dieses Produkt
SKU:
HA750524
Anwendung:
Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PS01-18
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Thr1989 of human ATR.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 301 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-ATR (T1989) on different lysates with Rabbit anti-Phospho-ATR (T1989) antibody (HA750524) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with Hydroxyurea cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750524) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300;000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells treated with or without 4mM Hydroxyurea for 20 hours labeling Phospho-ATR (T1989) with Rabbit anti-Phospho-ATR (T1989) antibody (HA750524) at 1/200 dilution. Image shown an increased nuclear staining upon Hydroxyurea treatment. Cells were fixed in 4% paraformaldehyde for 20 minutes at 37 ℃; permeabilized with 0.1% Triton X-100 in PBS permeabilization for 5 minutes; and then blocked with 2% negative goat serum for 60 minutes at room temperature. Cells were then incubated with Rabbit anti-Phospho-ATR (T1989) antibody (HA750524) at 1/200 dilution in 1% BSA overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.