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Antikörper

KDM3B Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750508-100UL
Über dieses Produkt
SKU:
HA750508
Anwendung:
Western Blot, IHC-Paraffin
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
1A5
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human KDM3B aa 251-500.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 191 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of KDM3B on different lysates with Rabbit anti-KDM3B antibody (HA750508) at 1/1;000 dilution. Lane 1: Hela cell lysate (30 µg/Lane) Lane 2: NIH/3T3 cell lysate (25 µg/Lane) Predicted band size: 191 kDa Observed band size: 191 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750508) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300;000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of A431 cells labeling KDM3B with Rabbit anti-KDM3B antibody (HA750508) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃; permeabilized with 0.05% Triton X-100 in PBS for 20 minutes; and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KDM3B antibody (HA750508) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunocytochemistry analysis of Hela cells labeling KDM3B with Rabbit anti-KDM3B antibody (HA750508) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃; permeabilized with 0.05% Triton X-100 in PBS for 20 minutes; and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-KDM3B antibody (HA750508) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-KDM3B antibody (HA750508) at 1/2;000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA750508) at 1/2;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.