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Antikörper

ACSL4/FACL4 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750469-100UL
Über dieses Produkt
SKU:
HA750469
Anwendung:
Western Blot, IHC-Paraffin
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
JE56-33
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human FACL4 aa 561-711 (Cytoplasmic).
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 79 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of ACSL4/FACL4 on different lysates with Rabbit anti-ACSL4/FACL4 antibody (HA750469) at 1/5;000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si ACSL4/FACL4 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750469) at 1/5;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of ACSL4/FACL4 on different lysates with Rabbit anti-ACSL4/FACL4 antibody (HA750469) at 1/1;000 dilution. Lane 1: PC-12 cell lysate Lane 2: C6 cell lysate Lane 3: L6 cell lysate Lane 4: Rat brain tissue lysate Lane 5: Rat lung tissue lysate Lane 6: Rat thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750469) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of ACSL4/FACL4 on different lysates with Rabbit anti-ACSL4/FACL4 antibody (HA750469) at 1/1;000 dilution. Lane 1: A549 cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 79 kDa Observed band size: 75 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750469) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ACSL4/FACL4 antibody (HA750469) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA750469) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.