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Antikörper

Phospho-ATM (S1981) Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750432-100UL
Über dieses Produkt
SKU:
HA750432
Anwendung:
Western Blot, IHC-Paraffin, Immunoprecipitation, Flow Cytometry
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
JM93-25
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Ser1981 of Human ATM aa 1;940-1;989 / 3;056.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 351 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-ATM (S1981) on different lysates with Rabbit anti-Phospho-ATM (S1981) antibody (HA750432) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 1μM Camptothecin for 1 hour cell lysate Lane 3: HeLa treated with 1μM Camptothecin for 1 hour cell lysate; then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 3 minutes; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750432) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-ATM (S1981) on different lysates with Rabbit anti-Phospho-ATM (S1981) antibody (HA750432) at 1/2;000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 20μM Etoposide for 2 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HEK-293 cell lysate Lane 5: MDA-MB-231 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750432) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells treated with or without 1μM Camptothecin for 1 hour labeling Phospho-ATM (S1981) with Rabbit anti-Phospho-ATM (S1981) antibody (HA750432) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-ATM (S1981) antibody (HA750432) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-ATM (S1981) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA750432; 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.