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Antikörper

Thymidylate Synthase Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750427-100UL
Über dieses Produkt
SKU:
HA750427
Anwendung:
Western Blot, IHC-Paraffin, Flow Cytometry, Immunoprecipitation
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
JM33-20
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human Thymidylate Synthase aa 264-313 / 313.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 36 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Thymidylate Synthase on different lysates with Rabbit anti-Thymidylate Synthase antibody (HA750427) at 1/1;000 dilution. Lane 1: NIH/3T3 cell lysate (20 µg/Lane) Lane 2: RAW264.7 cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: Mouse thymus tissue lysate (40 µg/Lane) Predicted band size: 36 kDa Observed band size: 35 kDa Exposure time: Lane 1-3: 3 minutes; Lane 4-5: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750427) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Thymidylate Synthase antibody (HA750427) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA750427) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Thymidylate Synthase antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded rat bone tissue using anti-Thymidylate Synthase antibody. Counter stained with hematoxylin.