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Antikörper

Argonaute 2 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750344-100UL
Über dieses Produkt
SKU:
HA750344
Anwendung:
Western Blot, IHC-Paraffin, Flow Cytometry, Immunoprecipitation
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
JF0992
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human Argonaute 2 aa 361-404 / 859.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 97 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
All lanes: Western blot analysis of Ago2 with anti-Ago2 antibody [JF0992] (HA750344) at 1:500 dilution. Lane 1: Wild-type CHO whole cell lysate. Lane 2: Ago2 knockout CHO whole cell lysate. ET1702-39 was shown to specifically react with Ago2 in wild-type CHO cells. No band was observed when Ago2 knockout samples were tested. Wild-type and Ago2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Ago2 antibody (ET1702-39; 1/500) and Anti-Vinculin antibody (ET1705-94; 1/5;000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200;000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co.; Ltd.; Guangzhou; China).
Western blot analysis of Argonaute 2 on different lysates with Rabbit anti-Argonaute 2 antibody (HA750344) at 1/2;000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 97 kDa Observed band size: 97 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750344) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HCT 116 cells labeling Argonaute 2 with Rabbit anti-Argonaute 2 antibody (HA750344) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Argonaute 2 antibody (HA750344) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.
Immunocytochemistry analysis of RAW264.7 cells labeling Argonaute 2 with Rabbit anti-Argonaute 2 antibody (HA750344) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Argonaute 2 antibody (HA750344) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.