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Antikörper

BTK Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750252-100UL
Über dieses Produkt
SKU:
HA750252
Anwendung:
Western Blot, IHC-Paraffin, Immunoprecipitation
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
SN69-07
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human BTK aa 610-659 / 659.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 76 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of BTK on different lysates with Rabbit anti-BTK antibody (HA750252) at 1/5;000 dilution. Lane 1: Ramos cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: Raji cell lysate Lane 4: THP-1 cell lysate Lane 5: Daudi cell lysate Lane 6: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 76 kDa Observed band size: 72 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750252) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
All lanes: Western blot analysis of BTK with anti-BTK antibody [SN69-07] (HA750252) at 1/1;000 dilution. Lane 1: Wild-type U2932 whole cell lysate. Lane 2: BTK knockout U2932 whole cell lysate. ET1611-4 was shown to specifically react with BTK in wild-type U2932 cells. No band was observed when BTK knockout sample was tested. Wild-type and BTK knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-BTK antibody (ET1611-4; 1/1;000) and Anti-Vinculin antibody (ET1705-94; 1/5;000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200;000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co.; Ltd.; Guangzhou; China).
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BTK antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA750252; 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.