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Antikörper

Active+Pro Caspase-9 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750238-100UL
Über dieses Produkt
SKU:
HA750238
Anwendung:
Western Blot, Immunoprecipitation
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
SC65-05
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Human Caspase-9 aa 95-335 / 416.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 46 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Active+Pro Caspase-9 on different lysates with Rabbit anti-Active+Pro Caspase-9 antibody (HA750238) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 1μM staurosporine for 4 hours cell lysate Lane 3: HeLa treated with 3μM staurosporine for 4 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 46/37/35 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750238) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Active+Pro Caspase-9 on different lysates with Rabbit anti-Active+Pro Caspase-9 antibody (HA750238) at 1/1;000 dilution. Lane 1: C2C12 cell lysate Lane 2: HeLa cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750238) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300;000 dilution was used for 1 hour at room temperature.
Western blot analysis of Caspase-9 on different lysates with Rabbit anti-Caspase-9 antibody (HA750238) at 1/1;000 dilution. Lane 1: Hela-si NT cell lysate Lane 2: Hela-si Caspase-9 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. ET1610-95 was shown to specifically react with Caspase-9 in Hela-si NT cells. Weakened band was observed when Hela-si Caspase-9 sample was tested. Hela-si NT and Hela-si Caspase-9 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-95; 1/1;000) and Loading control antibody (Rabbit anti-GAPDH; ET1601-4; 1/10;000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100;000 dilution was used for 1 hour at room temperature.