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Antikörper

ATM Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA750096-100UL
Über dieses Produkt
SKU:
HA750096
Anwendung:
Western Blot, IHC-Paraffin
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
SI70-01
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human ATM aa 1;951-2;000 / 3;056.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 351 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C or -80[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of ATM on different lysates with Rabbit anti-ATM antibody (HA750096) at 1/5;000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: NCCIT cell lysate Lane 4: HEK-293 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 24 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750096) at 1/5;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATM on different lysates with Rabbit anti-ATM antibody (HA750096) at 1/1;000 dilution. Lane 1: A549 cell lysate Lane 2: CRC cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 25 seconds; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750096) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATM on different lysates with Rabbit anti-ATM antibody (HA750096) at 1/2;000 dilution. Lane 1: A549 WT cell lysate Lane 2: A549 ATM KO cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 351 kDa Observed band size: 351 kDa Exposure time: 1 minute 10 seconds; ECL: Ori Supersensitive 4-20% SDS-PAGE gel. ET1606-20 was shown to specifically react with ATM in A549 WT cells. No band was observed when A549 ATM KO sample was tested. A549 WT and A549 ATM KO samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1606-20; 1/2;000) and Loading control antibody (Rabbit anti-GAPDH; ET1601-4; 1/10;000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:50;000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of HeLa cells labeling ATM with Rabbit anti-ATM antibody (HA750096) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ATM antibody (HA750096) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.