Skip to content

Warenkorb

Der Warenkorb ist leer.

Gesamt (exkl. MwSt.) £0,00
Warenkorb überprüfen & bezahlen
Antikörper

Human VCAM-1 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£449,00
HA725066B-100UL
Über dieses Produkt
SKU:
HA725066B
Anwendung:
ELISA
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol. Preservative: 0.05% ProClin300.
translate.label.attr.clone:
PSH12-47
Klonalität:
Monoclonal
Konjugat:
Biotin-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Human VCAM-1 aa 25-698 (HA210976).
Isotyp:
IgG
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Sandwich ELISA analysis of Human VCAM-1 matched pair antibodies Capture: HA725064; Human VCAM-1 Rabbit mAb [PSH12-46] Detector: HA725065; Human VCAM-1 Rabbit mAb [PSH12-47] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725064) diluted in carbonate/bicarbonate buffer; at a concentration of 2ug/ml overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer; and incubated with serial diluted Recombinant Human VCAM-1 protein (HA210976) protein starting from 10;000 pg/ml to 0 pg/ml and detect antibody (HA725065; Biotin; 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native VCAM-1 in human samples. Capture: HA725064; Human VCAM-1 Rabbit mAb [PSH12-46] Detector: HA725065; Human VCAM-1 Rabbit mAb [PSH12-47] Interpolated concentration of native VCAM-1 was measured in duplicate at different sample concentrations. Undiluted samples were 1% human serum. The interpolated dilution factor corrected values were plotted (mean +/- SD; n=2). The mean VCAM-1 concentration was determined to be 1;361;999 pg/mL in human serum. There was no detectable signal in SK-OV-3 cell culture supernatant.