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Antikörper

Human CXCL9 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£449,00
HA725006B-100UL
Über dieses Produkt
SKU:
HA725006B
Anwendung:
ELISA
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol. Preservative: 0.05% ProClin300.
translate.label.attr.clone:
PSH12-35
Klonalität:
Monoclonal
Konjugat:
Biotin-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Human CXCL9 aa 23-125 (HA210774).
Isotyp:
IgG
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Sandwich ELISA analysis of human CXCL9 matched pair antibodies Capture: HA725004; Human CXCL9 Rabbit mAb [PSH12-34] Detector: HA725005; Human CXCL9 Rabbit mAb [PSH12-35] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725004) diluted in carbonate/bicarbonate buffer; at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer; and incubated with serial diluted Recombinant Human CXCL9 protein (HA210774) starting from 4;000 pg/ml to 0 pg/ml and detect antibody (HA725005; Biotin; 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CXCL9 in THP-1 cell culture supernatant. Capture: HA725004; Human CXCL9 Rabbit mAb [PSH12-34] Detector: HA725005; Human CXCL9 Rabbit mAb [PSH12-35] THP-1 cells were stimulated with 200 ng/ml IFN-γ and 50ng/ml LPS or vehicle control and incubated for 24 hours. The concentrations of CXCL9 measured in duplicate and interpolated from the CXCL9 standard curve and corrected for sample dilution. Undiluted samples are as follows: unstimulated 100% and stimulated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD; n=2). The mean CXCL9 concentration was determined to be 841.6 pg/ml in IFN-γ and LPS stimulated THP-1 cell culture supernatant and undetectable in the unstimulated THP-1 control.