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Antikörper

Mouse IL-1 alpha Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA723526-100UL
Über dieses Produkt
SKU:
HA723526
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH13-40
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Mouse IL1 alpha aa 115-270 (HA210971).
Isotyp:
IgG
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Sandwich ELISA analysis of Mouse IL-1 alpha matched pair antibodies Capture: HA723526; Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527; Mouse IL-1 alpha Rabbit mAb [PSH13-41] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723526) diluted in carbonate/bicarbonate buffer; at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer; and incubated with serial diluted Recombinant Mouse IL-1 alpha protein (HA210971) starting from 1;200 pg/ml to 0 pg/ml and detect antibody (HA723527; Biotin; 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native IL-1alpha in Raw264.7 supernatant treated or untreated with LPS for 48 hours. Capture: HA723526; Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527; Mouse IL-1 alpha Rabbit mAb [PSH13-41] Interpolated concentration of native IL-1 alpha was measured in duplicate at different sample concentrations. Undiluted samples were 100% cell supernatant. The interpolated dilution factor corrected values were plotted (mean +/- SD; n=2). The mean IL-1 alpha concentration was determined to be 1;546 pg/mL in neat Raw264.7 treated supernatant; undetectable in untreated Raw264.7 supernatant.
Interpolated concentrations of spiked IL-1 alpha in cell culture media samples. Capture: HA723526; Mouse IL-1 alpha Rabbit mAb [PSH13-40] Detector: HA723527; Mouse IL-1 alpha Rabbit mAb [PSH13-41] The concentrations of IL-1 alpha were measured in duplicates; interpolated from the IL-1 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD; n=2).