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Antikörper

Human CCL17 / TARC Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA723516-100UL
Über dieses Produkt
SKU:
HA723516
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH13-35
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within Human CCL17 aa 24-94 (HA210895).
Isotyp:
IgG
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Sandwich ELISA analysis of Human CCL17 / TARC matched pair antibodies Capture: HA723513; Human CCL17 / TARC Rabbit mAb [PSH13-33] Detector: HA723516; Human CCL17 / TARC Rabbit mAb [PSH13-35] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723513) diluted in carbonate/bicarbonate buffer; at a concentration of 5 µg/mL overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer; and incubated with serial diluted Recombinant Human CCL17 / TARC protein (HA210895) starting from 2;000 pg/ml to 0 pg/ml and detect antibody (HA723516; Biotin; 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Interpolated concentrations of native CCL17 in human PBMC cell culture supernatant. Capture: HA723513; Human CCL17 / TARC Rabbit mAb [PSH13-33] Detector: HA723516; Human CCL17 / TARC Rabbit mAb [PSH13-35] PBMC cells were stimulated with 10 µg/ml PHA-M and incubated for 5 days. The concentrations of CCL17 measured in duplicate and interpolated from the CCL17 standard curve and corrected for sample dilution. Undiluted samples are as follows: stimulated 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD; n=2). The mean CCL17 concentration was determined to be 8;300 pg/ml in PHA-M stimulated PBMC cell culture supernatant.
Interpolated concentrations of spiked CCL17 in human cell culture media samples. Capture: HA723513; Human CCL17 / TARC Rabbit mAb [PSH13-33] Detector: HA723516; Human CCL17 / TARC Rabbit mAb [PSH13-35] The concentrations of CCL17 were measured in duplicates; interpolated from the CCL17 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD; n=2).