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Antikörper

COL5A1 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£425,00
HA723177-100UL
Über dieses Produkt
SKU:
HA723177
Anwendung:
Western Blot, Flow Cytometry, Immunoprecipitation
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
PSH10-08
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human COL5A1 aa 251-450.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 184 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of COL5A1 on Saos-2 cell lysates with Rabbit anti-COL5A1 antibody (HA723177) at 1/2;000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 184 kDa Observed band size: 250 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723177) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of Saos-2 cells labeling COL5A1 with Rabbit anti-COL5A1 antibody (HA723177) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-COL5A1 antibody (HA723177) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488; HA1121) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.
Flow cytometric analysis of Saos-2 cells labeling COL5A1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA723177; 1/1;000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour; the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1;000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
COL5A1 was immunoprecipitated from 0.2 mg Saos-2 cell lysate with HA723177 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723177 at 1/1;000 dilution. Mouse anti Rabbit IgG heavy chain (Fc) secondary antibody (M1003-7) at 1/10;000 dilution was used for 1 hour at room temperature. Lane 1: Saos-2 cell lysate (input) Lane 2: HA723177 IP in Saos-2 cell lysate Lane 3: Rabbit IgG instead of HA723177 in Saos-2 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 20 seconds; ECL: K1801