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Antikörper

ATF-4 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£425,00
HA722796-100UL
Über dieses Produkt
SKU:
HA722796
Anwendung:
Western Blot, Immunoprecipitation
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
PSH07-08
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within human ATF-4 aa 1-300.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 39 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of ATF-4 on different lysates with Rabbit anti-ATF-4 antibody (HA722796) at 1/2;000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: HEK-293 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: C6 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Predicted band size: 39 kDa Observed band size: 50 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722796) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of ATF-4 on different lysates with Rabbit anti-ATF-4 antibody (HA722796) at 1/2;000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: HeLa treated with 250μM CoCl2 for 6 hours cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: NIH/3T3 treated with 2μg/mL Tunicamycin for 8 hours cell lysate (20 µg/Lane) Predicted band size: 39 kDa Observed band size: 50 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722796) at 1/2;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
ATF-4 was immunoprecipitated from 0.2 mg HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate with HA722796 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722796 at 1/1;000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5;000 dilution was used for 1 hour at room temperature. Lane 1: HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate (input) Lane 2: HA722796 IP in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate Lane 3: Rabbit IgG instead of HA722796 in HeLa treated with 2μg/mL Tunicamycin for 8 hours cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 40 seconds; ECL: K1802