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Antikörper

AMPK gamma 1 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£425,00
HA722691-100UL
Über dieses Produkt
SKU:
HA722691
Anwendung:
Western Blot, Immunoprecipitation
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE07-47
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within human AMPK gamma 1 aa 282-331 / 331.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 38 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of AMPK gamma 1 on different lysates with Rabbit anti-AMPK gamma 1 antibody (HA722691) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722691) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Western blot analysis of AMPK gamma 1 on different lysates with Rabbit anti-AMPK gamma 1 antibody (HA722691) at 1/1;000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722691) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
AMPK gamma 1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722691 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722691 at 1/1;000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5;000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA722691 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA722691 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801