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Antikörper

TRPM7 Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£425,00
HA721501-100UL
Über dieses Produkt
SKU:
HA721501
Anwendung:
Western Blot, Flow Cytometry
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE38-36
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within Human TRPM7 aa 1;816-1;865 / 1;865.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 213 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of TRPM7 on different lysates with Rabbit anti-TRPM7 antibody (HA721501) at 1/1;000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HepG2 cell lysate Lane 4: HCT 116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 213 kDa Observed band size: 250 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721501) at 1/1;000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100;000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of HeLa cells labeling TRPM7. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA721501; 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour; the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1;000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).