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Antikörper

Phospho-HER2 / ErbB2 (Y1221 + Y1222) Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£425,00
HA721433-100UL
Über dieses Produkt
SKU:
HA721433
Anwendung:
Western Blot
Buffer:
PBS (pH7.4); 0.1% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE44-12
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding tyrosines 1221/1222 of human ErbB2 protein
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 138 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of Phospho-HER2 / ErbB2 (Y1221 + Y1222) on different lysates with Rabbit anti-Phospho-HER2 / ErbB2 (Y1221 + Y1222) antibody (HA721433) at 1/1;000 dilution. Lane 1: SK-Br-3 whole cell lysate Lane 2: SK-Br-3 treated with λpp for 1 hour whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 138 kDa Observed band size: 250 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721433) at 1/1;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100;000 dilution was used for 1 hour at room temperature.