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Antikörper

Human IL-1 beta Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£689,00
HA721279-100UL
Über dieses Produkt
SKU:
HA721279
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PS00-82
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Recombinant protein within full length human IL-1 beta.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 31 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
The binding activity of IL-1 beta (HA721279) with recombinant IL-1 beta protein. Immobilized recombinant IL-1 beta protein at 1 μg/ml overnight at 4℃. Then blocked with 1xTBS/1%BSA for 1 hour at 37℃; and incubated with the primary antibody (HA721279) for 45min at 37℃. Then the plate was washed and incubated with 50 µl per well of Goat anti-Rabbit IgG-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Standard curve of IL-1 beta matched pair antibodies: Sandwich ELISA analysis of IL-1 beta matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody HA721278 [PS01-39] diluted in carbonate/bicarbonate buffer; at a concentration of 4 µg/mL overnight at 4℃. Wells of the plate were washed; blocked with 150 µl 1% BSA/PBST blocking buffer; and incubated with serial diluted recombinant IL-1 beta protein starting from 2000 pg/ml to 31.25 pg/ml for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of detect antibody [PS00-82] (Biotin; 1:2;000) for 1 hour at 37℃. Then the plate was washed and incubated with 50 µl per well of Streptavidin-HRP for 0.5 hour at 37℃. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.