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Antikörper

AAMP Recombinant Rabbit monoclonal Antibody

Produktgrößen
100μl
£425,00
HA721054-100UL
Über dieses Produkt
SKU:
HA721054
Anwendung:
Western Blot, Flow Cytometry
Buffer:
1*TBS (pH7.4); 0.05% BSA; 40% Glycerol. Preservative: 0.05% Sodium Azide.
translate.label.attr.clone:
JE64-70
Klonalität:
Monoclonal
Konjugat:
Non-conjugated
Host:
Rabbit
Immunogen:
Synthetic peptide within human AAMP aa 2-51/434.
Isotyp:
IgG
Molekulargewicht:
Predicted band size: 47 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Shipped at 4[o]C. Store at +4[o]C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20[o]C long term.
Hersteller:
Huabio
Typ:
Antibodies: Monoclonal Antibody
Western blot analysis of AAMP on different lysates with Rabbit anti-AAMP antibody (HA721054) at 1/2;000 dilution. Lane 1: HepG2 cell lysate; 10 µg/Lane Lane 2: 293T cell lysate; 10 µg/Lane Lane 3: Rat testis tissue lysate; 20 µg/Lane Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721054) at 1/2;000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300;000 dilution was used for 1 hour at room temperature.
Western blot analysis of AAMP on different lysates with Rabbit anti-AAMP antibody (HA721054) at 1/500 dilution. Lane 1: MCF-7 cell lysate; 10 µg/Lane Lane 2: HUVEC cell lysate; 10 µg/Lane Lane 3: NIH/3T3 cell lysate; 10 µg/Lane Lane 4: Rat brain tissue lysate; 20 µg/Lane Predicted band size: 47 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721054) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300;000 dilution was used for 1 hour at room temperature.
Flow cytometric analysis of HepG2 cells labeling AAMP. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721054; 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour; the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1;000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).