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Antikörper

Cytokeratin 19 Recombinant Chimeric Antibody Antibody

Produktgrößen
100μl
£425,00
HA610370-100UL
Über dieses Produkt
SKU:
HA610370
Anwendung:
Western Blot, IHC-Paraffin, Flow Cytometry
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
SA30-06
Konjugat:
Non-conjugated
Immunogen:
Synthetic peptide within Human Cytokeratin 19 aa 348-400 / 400.
Molekulargewicht:
Predicted band size: 44 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies
Western blot analysis of Cytokeratin 19 on different lysates with Rat anti-Cytokeratin 19 antibody (HA610370) at 1/5;000 dilution. Lane 1: MCF7 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: T-47D cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: Lane 1-3: 4 seconds; Lane 4: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610370) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunocytochemistry analysis of MCF7 cells labeling Cytokeratin 19 with Rat anti-Cytokeratin 19 antibody (HA610370) at 1/10;000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-Cytokeratin 19 antibody (HA610370) at 1/10;000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor™ 488; HA1133) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594; HA1126) was used as the secondary antibody at 1/1;000 dilution.
Immunocytochemistry analysis of PC-12 cells labeling Cytokeratin 19 with Rat anti-Cytokeratin 19 antibody (HA610370) at 1/1;000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature; permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature; then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-Cytokeratin 19 antibody (HA610370) at 1/1;000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor™ 488; HA1133) was used as the secondary antibody at 1/1;000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4; red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594; HA1122) were used as the secondary antibody at 1/1;000 dilution.
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rat anti-Cytokeratin 19 antibody (HA610370) at 1/1;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610370) at 1/1;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.