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Antikörper

CD20 Recombinant Chimeric Antibody Antibody

Produktgrößen
100μl
£425,00
HA610360-100UL
Über dieses Produkt
SKU:
HA610360
Anwendung:
Western Blot, IHC-Paraffin, IHC-Frozen
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PD00-02
Konjugat:
Non-conjugated
Immunogen:
Synthetic peptide within Human CD20 aa 210-297 (Cytoplasmic).
Molekulargewicht:
Predicted band size: 33 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies
Western blot analysis of CD20 on different lysates with Mouse anti-CD20 antibody (HA610360) at 1/5;000 dilution. Lane 1: Raji cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (negative) (20 µg/Lane) Lane 3: Ramos cell lysate (20 µg/Lane) Lane 4: Daudi cell lysate (20 µg/Lane) Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610360) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1001) at 1/50;000 dilution was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-CD20 antibody (HA610360) at 1/20;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610360) at 1/20;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD20 antibody (HA610360) at 1/20;000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature; washed with ddH2O and PBS; and then probed with the primary antibody (HA610360) at 1/20;000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application: IHC-Fr Species: Mouse Site: Spleen Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required