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Antikörper

Glutamine Synthetase Recombinant Chimeric Antibody Antibody

Produktgrößen
100μl
£425,00
HA610346-100UL
Über dieses Produkt
SKU:
HA610346
Anwendung:
IHC-Frozen, Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH10-48
Konjugat:
Non-conjugated
Isotyp:
IgY
Molekulargewicht:
Predicted band size: 42 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies
Application: IHC-Fr Species: Mouse Site: bran (P7) Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Recommend. The section was pre-treated using 1% SDS buffer (in PBS; pH 7.4) for 5 minutes at room temperature.
Western blot analysis of Glutamine Synthetase on different lysates with Chicken anti-Glutamine Synthetase antibody (HA610346) at 1/5;000 dilution. Lane 1: Mouse brain tissue lysate (10 µg/Lane) Lane 2: Mouse liver tissue lysate (10 µg/Lane) Lane 3: Mouse kidney tissue lysate (10 µg/Lane) Lane 4: Rat brain tissue lysate (10 µg/Lane) Lane 5: Rat liver tissue lysate (10 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610346) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50;000 dilution was used for 1 hour at room temperature.