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Antikörper

EAAT2 Recombinant Chimeric Antibody Antibody

Produktgrößen
100μl
£425,00
HA610334-100UL
Über dieses Produkt
SKU:
HA610334
Anwendung:
IHC-Frozen, Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PS01-62
Konjugat:
Non-conjugated
Immunogen:
Synthetic peptide within Human EAAT2
Molekulargewicht:
Predicted band size: 62 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies
Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
Western blot analysis of EAAT2 on different lysates with Guinea pig anti-EAAT2 antibody (HA610334) at 1/5;000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Mouse lung tissue lysate (negative) (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610334) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/50;000 dilution was used for 1 hour at room temperature.