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Antikörper

Doublecortin Recombinant Chimeric Antibody Antibody

Produktgrößen
100μl
£425,00
HA610325-100UL
Über dieses Produkt
SKU:
HA610325
Anwendung:
IHC-Frozen, Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
JJ0959
Konjugat:
Non-conjugated
Immunogen:
Synthetic peptide within Human Doublecortin aa 22-71 / 365.
Isotyp:
IgY
Molekulargewicht:
Predicted band size: 41 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies
Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
Western blot analysis of Doublecortin on different lysates with Chicken anti-Doublecortin antibody (HA610325) at 1/10;000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane) Lane 2: A549 cell lysate (negative) (15 µg/Lane) Lane 3: Mouse brain (P0) tissue lysate (20 µg/Lane) Lane 4: Mouse brain (P60) tissue lysate (20 µg/Lane) Lane 5: Mouse brain (P60) tissue lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 41/45 kDa Exposure time: 2 minutes 40 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610325) at 1/10;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50;000 dilution was used for 1 hour at room temperature.