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Antikörper

GAD65 Recombinant Chimeric Antibody Antibody

Produktgrößen
100μg
£689,00
HA610307-100UG
Über dieses Produkt
SKU:
HA610307
Anwendung:
IHC-Frozen, Western Blot
Buffer:
PBS (pH7.4).
translate.label.attr.clone:
PSH06-80
Konjugat:
Non-conjugated
Isotyp:
IgY
Molekulargewicht:
Predicted band size: 65 kDa
Physischer Zustand:
Liquid
Reinheit:
Protein A affinity purified.
Versandbedingungen:
Blue Ice
Lagerbedingungen:
Store at +4[o]C after thawing. Aliquot store at -20[o]C. Avoid repeated freeze / thaw cycles.
Hersteller:
Huabio
Typ:
Antibodies
Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
Western blot analysis of GAD65 on different lysates with Chicken anti-GAD65 antibody (HA610307) at 1/5;000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610307) at 1/5;000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50;000 dilution was used for 1 hour at room temperature.